Rat A fetal globulin/alpha-fetoprotein (AFP) elisa kit instruction manual

Rat a fetal globulin/alpha-fetoprotein (AFP) elisa kit Instructions for use Elisa kit Specifications: 48-well configuration / 96-well configuration Standard dilution: 1.5ml × 1 bottle of enzyme standard reagent: 3 ml × 1 bottle ( 48)/6 ml×1 bottle (96) [rat alpha-fetoprotein/a-fetoprotein (AFP) elisa kit] This reagent is for research use only: the concentration of the standard is the abscissa, and the OD value is On the ordinate, draw a standard curve on the coordinate paper, find the corresponding concentration from the standard curve according to the OD value of the sample; multiply by the dilution factor; or calculate the linear regression equation of the standard curve by using the concentration of the standard and the OD value, Substituting the OD value of the sample into the equation, calculating the sample concentration, and multiplying by the dilution factor, is the actual concentration of the sample. Kit composition: sealing film: 2 pieces (48) / 2 pieces (96) Instructions: 1 part sealed bag: 1 standard: 2700ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation enzyme Standard package: 1×48 1×96 2-8°C Preservation of sample dilution: 3ml×1 bottle 6 ml×1 bottle 2-8°C Preservation of developer A: 3ml×1 bottle 6 ml×1 bottle 2 Store chromogen B solution at -8 °C: 3ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution: 3ml × 1 bottle 6ml × 1 bottle 2-8 ° C Preservation concentrated washing solution: (20ml × 20 times) ×1 bottle (20ml×30 times)×1 bottle 2-8°C preservation experiment principle: This kit uses the double antibody sandwich method to determine the level of rat fetal globulin/alpha-fetoprotein (AFP) in the specimen. The microplate was coated with purified rat alpha fetoprotein/alpha-fetoprotein (AFP) antibody to prepare a solid phase antibody, and the mouse alpha fetoprotein/alpha-fetoprotein was sequentially added to the microcapsule of the coated monoclonal antibody. (AFP), and then combined with HRP-labeled murine alpha-fetoprotein/alpha-fetoprotein (AFP) antibody to form an antibody-antigen-enzyme-labeled antibody complex, which was thoroughly washed and then added with substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with the mouse fetoprotein/alpha-fetoprotein (AFP) in the sample. The absorbance (OD value) was measured by a microplate reader at a wavelength of 450 nm, and the concentration of rat alpha fetoprotein/alpha-fetoprotein (AFP) in the sample was calculated from a standard curve.

Heat Shrinkable Tape

Heat Shrinkable Tape

I System description:

Heat Shrinkable Tape is designed to protect the weld joint of buried or exposed steel pipelines and insulated pipeline. This sleeves are manufactured from high quality cross linked polyethylene material. Hot melt adhesive provides excellent adhesion with the surface of steel pipeline, PE coating. Meanwhile it forms a continuous and firm anti-corrosive system with the surface of the pipeline.

The Polyethylene Heat shrinkable Tape needs no primer nor patch closure and reduces application time and labour costs.

â…¡ Structure

The specification of the tape consists of three layers,

Adhesive: butyl rubber based hot melt adhesive

Film backing: Radiation crosslinked polyethylene

Interleaf:Antiadhesive Film Treated with Silicone

â…¢ Features

After heating, formed corrosion protection seal

Strong backing provides outstanding waterproofing effect

High chemical resistance. resists impact, abrasion and ultraviolet light.

Excellent resistance against cathodic disbondment, dielectric breakdown.

Simple Application

â…£ Physical Properties

· Product Data Sheet Total Thickness:40mil, 50 mil,60 mil ,65 mils.

· Width:50,100,150,300,400,500,800 mm

· Length: 10 m

· Tensile Strength:35 N/cm

· Elongation:400%

· Adhesion Strength to pipe:30 N/cm

· Application temperature: -10 -60 ℃

· Temperature resistance:-50 ℃ - 80℃

· Shelf Life: 12 months

â…¤ Application

No Special skills, equipments, patch closure nor primer are needed. Its superior flexibility provides easy and reliable application for irregular configurations of fittings, bends and elbows and small diameter of straight pipes

Wrap spirally the tape over foreign matters-free and preheated surface, beginning at least 25~50mm (1~2 ) onto pipe coating. Wrap the tape with enough tension to obtain conformability and with minimum 50% of overlapping, while removing the release liner. Warm the end part of the tape and press down firmly. Using a torch, start at the edge of the tape and heat circumferentially around the coated pipe.

Finish off by heating over the entire tape vertically to ensure a uniform adhesion and by using a roller or gloved hands to remove wrinkle and air.

VI. Technical Datas


Heat Shrinkable Tape, Shrink Wrap Tape, Heat Applied Tape

Jining Xunda Pipe Coating Materials Co.,Ltd , https://www.pipe-wrap.com