Recently, a scientific research team led by Shi Zhengli, a researcher at the Institute of Virology, Chinese Academy of Sciences, used second-generation high-throughput sequencing technology to systematically analyze the transcriptional spectrum of bat adenovirus BtAdV-TJM infected bat cell lines, providing a complete set of bat glands The dynamic distribution of viral gene expression during viral replication. The results were published in the first issue of Journal of Virology in 2013.
Adenovirus is widespread in vertebrates including humans and non-human primates, and can cause respiratory and intestinal diseases in humans. Due to its good immunogenicity, adenovirus has been developed as a carrier for vaccines and gene therapy. However, the infection of human adenovirus has restricted the wide application of human adenovirus as a gene carrier. Therefore, it is necessary to develop other animal adenoviruses as new vectors.
In the early stage of this research group, a new type of bat adenovirus (BtAdV-TJM) was isolated from primary bat cells (Li et al., JVI, 2010). In this study, bat adenovirus was used to infect David's ear bat cell line, and high-throughput sequencing technology was used to systematically study the transcriptome of the virus and host cells. The results showed that with the increase of infection time, the transcriptional replication of bat adenovirus in bat cells accelerated, reaching a peak 18h after infection. Similar to human adenovirus infection, bat adenovirus replication is divided into three stages: early, middle and late. Early genes mainly encode some proteins that interact with the host, mid-term genes mainly encode proteins related to self-replication, and late genes encode viral assembly and structural proteins. RT-PCR confirmed that 7 bat adenovirus genes are transcribed and replicated differently from human adenovirus. This indicates that the transcriptional replication mechanism of bat adenovirus may be different from that of human and other mammalian sources. At the same time, this study functionally classified the up- and down-regulated genes of host cells after virus infection, which were mainly divided into cellular immune response, transcription, translation, cell replication and repair, among which 79 host immune genes were significantly up-regulated after virus infection. The above research results provide the basis for the development of bat adenovirus vectors and the study of the bat immune system's antiviral immune response.
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