Human elisa, apelin 13 (AP13) kit sample processing Guangrui Bio-domestic high and excellent Elisa kit supplier Sample processing and requirements: 1. Serum: Room temperature blood coagulation for 10-20 minutes, centrifuged for about 20 minutes (2000- 3000 rpm). Carefully collect the supernatant and if sedimentation occurs during storage, centrifuge it again. 2. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After 10-20 minutes of mixing, centrifuge for 20 minutes (2000-3000 r/min). Carefully collect the supernatant and if any precipitate forms during storage, it should be centrifuged again. 3. Urine: Collect with a sterile tube and centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again. Pleural fluid, cerebrospinal fluid reference implementation. 4. Cell culture supernatant: When detecting secretory components, use a sterile tube for collection. Centrifugation for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When detecting intracellular components, the cell suspension was diluted with PBS (pH 7.2-7.4) to a cell concentration of about 1 million cells/ml. By repeated freezing and thawing, the cells are destroyed and the intracellular components are released. Centrifugation for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If precipitation forms during storage, it should be centrifuged again. 25. Tissue specimen: Weigh the specimen after cutting it. Add a certain amount of PBS, pH 7.4. Liquid nitrogen quickly frozen for use. After the sample melts, it still maintains a temperature of 2-8°C. A certain amount of PBS (pH 7.4) was added and the sample was homogenized by hand or homogenizer. Centrifugation for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. One part is to be tested after dispensing and the rest is frozen for use. 6. Samples should be extracted as soon as possible after collection. Extraction should be carried out according to the relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20°C, but repeated freezing and thawing should be avoided. 7. The NaN3-containing sample cannot be detected because NaN3 inhibits horseradish peroxidase (HRP) activity.