The spermatogonial stem cells were taken from the cynomolgus monkey body, and cultured to induce the transformation into sperm cells, spermatocytes, in vitro. This leading technology builds a public research platform for in vitro studies of spermatogenesis, treatment of male infertility, regenerative medicine research, and clinical applications. Recently, the Hainan Provincial People's Hospital announced this good news.
This research is a project funded by the Hainan Natural Science Foundation. It was completed by the Central Laboratory of Hainan Provincial People's Hospital and the Laboratory of Mammal Reproduction and Biotechnology of Inner Mongolia University. It has been 3 years since the project started in August 2010, and the subculture of cynomolgus monkey spermatogonial stem cells has reached 22 generations. After analysis by immunofluorescence dual-label staining, the expression of spermatogonial stem cell marker genes remained unchanged. The results of in vitro differentiation experiments show that long-term cultured cynomolgus monkey spermatogonial stem cells have physiological functions.
Studies have shown that long-term cultured spermatogonial stem cells can restore fertility in infertile animals through testicular transplantation. Researched a successful technology in Hainan, extending the cynomolgus monkey spermatogonial stem cells from the isolation and purification stage to the subculture and freezing storage stages, and established an immunofluorescence double-labeled staining method for identifying cynomolgus monkey spermatogonial stem cells, which is clearly ahead of home and abroad Similar research.
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