Elisa kit standard curve drawing method

How to draw a standard curve? Various mapping software can be used to draw the ELISA standard curve. The following is the example of "CurveExert1.3" software. The method of drawing the ELISA standard curve is as follows: 1 Start "CurveExpert1.3" 2. The X-axis input standard OD value, Y-axis input corresponding to the concentration value, as shown in the figure: 3. Click the [Run] button and the following dialog box appears. Click the [ok] button, the following two dialog boxes appear, close the next dialog box. 5. In the upper right corner of the dialog box, some curve names appear. Click on the curve name from the "1" and the corresponding figure appears in the lower right corner. The curve of the combination. 6. Select the curve with the best ELISA fit according to the fitted curve. Double-click the following dialog box: Note: Select the best curve equation for the coefficient (ie “r” value) to perform the operation. In the upper right corner of the dialog box below, there is an "r" value. When the value of "r" is closer to 1 the better the degree of fit. Press [Ctrl] + [L] to display the following dialog box: 8. Enter the standard OD value and click [Calculate] to press 忸 to get the actual content of the protein to be tested. (The specimen is diluted N times and the calculated value should be in N). 9. If you want to get the equation of the ELISA fit curve, you can right-click in the blank space of step 6 and select “Information”. 10. Get the following dialog box: Click “Copy” to paste the location you need to get the following data: RationalFunction: y=(a+bx)/(1+cx+dx^2) CoefficientData: a=-21.531017 b=212.05019 c=-0.24054479 d=0.018060778

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